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Protein Biochemistry
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Fluorophores, Dyes & Probes
Fluorophores and Dyes
7-AAD Viability Staining Solution
7-AAD Viability Staining Solution from BioLegend
(6)
Write a review
Citations:
(439)
Supplier Page
Supplier Page from
BioLegend for
7-AAD Viability Staining Solution
Description
7-AAD Viability Staining Solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
7-AAD Viability Staining Solution
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
420404
Applications
Flow Cytometry (FC)
Quantity
500 tests
Format
PBS, pH 7.2 with 0.09% (w/v) sodium azide
Target
7-AAD
Concentration
50.00 µg/mL
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (439)
(41)
Microparticle-Delivered Cxcl9 Prolongs Braf Inhibitor Efficacy in Melanoma
Cancer Immunology Research
May 3, 2023
Gabriele Romano, Francesca Paradiso, Peng Li, Pooja Shukla, Lindsay N. Barger, Olivia El El Naggar, John P. Miller, Roger J. Liang, Timothy L. Helms, Alexander J. Lazar,
et al.
(
clone
PC61).
7-AAD Viability Staining Solution
was used following the manufacturer’s instructions (#
420403
,
Biolegend
). Guava easyCyte 11HT Benchtop
Flow Cytometer
was utilized for the acquisition, and results
(42)
OSMR deficiency aggravates pressure overload-induced cardiac hypertrophy by modulating macrophages and OSM/LIFR/STAT3 signalling
Journal of translational medicine
April 29, 2023
Feng Y, Yuan Y, Xia H, Wang Z, Che Y, Hu Z, Deng J, Li F, Wu Q, Bian Z,
et al
.
dark (Additional file 1: Table S1). Dead cells were stained with
7-AAD Viability Staining Solution
(#
420404
,
BioLegend
, USA) 5 min before the
assay
. Data were acquired on a BD FACS Arial II
flow cytometer
(BD
(43)
The Central Nervous System Source Modulates Microglia Function and Morphology In Vitro
International Journal of Molecular Sciences
April 22, 2023
Andreia G. Pinho, Andreia Monteiro, Sara Fernandes, Nídia de Sousa, António J. Salgado, Nuno A. Silva, Susana Monteiro
FSC-A versus FSC-H, and dead cells were excluded by positive
staining
for
7-AAD
(
BioLegend
, Cat. No.
420403
) (Supplementary Figure S1).
Microglial cells
were gated according to
CD11b
+ expression (Supplementary
(44)
MicroRNA-708 emerges as a potential candidate to target undruggable NRAS
PLOS ONE
April 21, 2023
Jia Meng Pang, Po-Chen Chien, Ming-Chien Kao, Pei-Yun Chiu, Pin-Xu Chen, Yu-Ling Hsu, Chengyang Liu, Xiaowei Liang, Kai-Ti Lin
collected and double stained with 80 ng/mL Annexin V (
BioLegend
,
640920
) and 5 μg/mL 7-AAD (
BioLegend
,
420403
) in calcium-rich binding buffer (10mM pH 7.8 HEPES, 140mM NaCl, 2.5mM CaCl2) for 15 minutes. The stained
(45)
Induction of unique macrophage subset by simultaneous stimulation with LPS and IL-4
Frontiers in Immunology
April 21, 2023
Kei Ishida, Takahiro Nagatake, Azusa Saika, Soichiro Kawai, Eri Node, Koji Hosomi, Jun Kunisawa
Violet
(BV) 421-anti-
CD45
(
1:100
;
103133
,
BioLegend
). Dead cells were detected by using 7-AAD (
1:100
;
420404
,
BioLegend
) and excluded from analysis.
Flow cytometric analysis
was conducted by using
FACSAria
(
BD Biosciences
(46)
Liver X Receptor Activation Attenuates Oxysterol-Induced Inflammatory Responses in Fetoplacental Endothelial Cells
Cells
April 19, 2023
Meekha George, Magdalena Lang, Chaitanya Chakravarthi Gali, Joshua Adekunle Babalola, Carmen Tam-Amersdorfer, Anika Stracke, Herbert Strobl, Robert Zimmermann, Ute Panzenboeck, Christian Wadsack
examined with the cytometer. Dead cells were excluded from analysis using 7-Amino Actinomycin D (7-AAD) (
420403
;
Biolegend
, San Diego, CA, USA) cell viability dye that binds specifically to dead cells. Doublet exclusion
(47)
The cholinergic anti-inflammatory pathway inhibits inflammation without lymphocyte relay
Frontiers in Neuroscience
April 14, 2023
Thomas Simon, Joseph Kirk, Nikola Dolezalova, Mélanie Guyot, Clara Panzolini, Alexandre Bondue, Julien Lavergne, Sandrine Hugues, Nicolas Hypolite, Kourosh Saeb-Parsy,
et al.
(
Abcam
, cat. no.
ab150073
) was added, together with the viability stain 7-AAD (
BioLegend
, cat. no.
420404
) and the remaining conjugated antibodies [
CD19
PE (
BioLegend
, cat. no.
363004
), CD14 PE-Vio770 (Miltenyi
(48)
Coordinated activation of c-Src and FOXM1 drives tumor cell proliferation and breast cancer progression
The Journal of clinical investigation
April 3, 2023
Nandi I, Smith HW, Sanguin-Gendreau V, Ji L, Pacis A, Papavasiliou V, Zuo D, Nam S, Attalla SS, Kim SH,
et al
.
for 1 hour at room temperature, and incubated with 5 μg/mL 7-amino-actinomycin D (
7-AAD
) (
BioLegend
,
420403
) in FACS buffer to stain DNA. EdU incorporation was detected using the Invitrogen Click-iT EdU Alexa
(49)
Three‐dimensional chromatin re‐organization during muscle stem cell aging
Aging Cell
April 1, 2023
Benjamin A. Yang, Jacqueline A. Larouche, Kaitlyn M. Sabin, Paula M. Fraczek, Stephen C. J. Parker, Carlos A. Aguilar
,
Sigma Aldrich
,
492018‐50ML
Sigma protector RNase inhibitor,Sigma Aldrich,3335402001 DL‐DTT,
Sigma Aldrich
,
646563
7‐AAD,
Biolegend
,
420403
MACS BSA stock solution
,
Miltenyi
,
130‐091‐376
"
New England Biolabs
, Inc. supplier diversity partner
(50)
Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities
bioRxiv : the preprint server for biology
March 29, 2023
Garg V, Yang Y, Nowotschin S, Setty M, Kuo YY, Sharma R, Polyzos A, Salataj E, Murphy D, Jang A,
et al
.
proteins" Hoechst,
Invitrogen
,Cat#
H3570
"4’,6-diamidino-2-phenylindole (DAPI)",
Invitrogen
,Cat#
D1306
7-AAD viability dye,
BioLegend
,Cat#
420404
Gelatin,
Millipore Sigma
,Cat#
G9391
RPMI 1640 medium
,
Gibco
,Cat#
11875093
DMEM medium,
Gibco
,Cat#
11995073
Fetal
(51)
Targeting Tn-positive tumors with an afucosylated recombinant anti-Tn IgG
Scientific Reports
March 28, 2023
Yasuyuki Matsumoto, Nan Jia, Jamie Heimburg-Molinaro, Richard D. Cummings
1:400
in PBS) on a
flow cytometer
. For CDC assay, cells were stained with 7-Aminoactinomycin D (Cat#
420403
, 7-AAD,
BioLegend
, diluted at
1:100
in PBS) for 10 min at RT in the dark. Dead and viable cells were
(52)
A combination therapy of bortezomib, CXCR4 inhibitor, and checkpoint inhibitor is effective in cholangiocarcinoma in vivo
iScience
March 17, 2023
Li L, Zhou Y, Zhang Y, Hu H, Mao HQ, Selaru FM.
peptides and recombinant proteins","Chemicals, peptides and recombinant proteins" ,, 7-ADD,
BioLegend
,Cat#
420403
collagenase 1A,MilliporeSigma,Cat#C9891 DNase,MilliporeSigma,Cat#9003-98-9 33%/77% percoll,
MilliporeSigma
,Cat#
P4937
Bortezomib
,
Selleckchem
,Cat#
S1013
Plerixafor,
Selleckchem
,Cat#
S8030
RIPA
(53)
WFDC12-overexpressing contributes to the development of atopic dermatitis via accelerating ALOX12/15 metabolism and PAF accumulation
Cell death & disease
March 8, 2023
Li G, Gu L, Zhao F, Hu Y, Wang X, Zeng F, Yu J, Yue C, Zhou P, Li Y,
et al
.
min. The cellular viability was assessed by
staining
with 7-amino actinomycin D (7-AAD) (
BioLegend
,
420404
; 0.5 μg/mL) to exclude dead cells. For the analysis of IL-4、IFN-γ and IL-17A production,
in vitro
re-stimulation,
(54)
Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart
npj Regenerative Medicine
March 3, 2023
Panagiota Siatra, Giannis Vatsellas, Athanasia Chatzianastasiou, Evangelos Balafas, Theodora Manolakou, Andreas Papapetropoulos, Anna Agapaki, Eleni-Taxiarchia Mouchtouri, Prashant J. Ruchaya, Artemis G. Korovesi,
et al.
anti-
mouse
KDR
-Alexa647 conjugated with APC (BioLegend, Cat no.
121910
). 7-AAD (
BioLegend
, Cat no.
420404
) was used as a viability marker.
Sca-1
(
Biolegend
Cat no.
108127
), c-Kit (Biolegend, Cat no.
105813
),
(55)
A theoretical framework of immune cell phenotypic classification and discovery
Frontiers in Immunology
March 2, 2023
Yuzhe Hu, Chen Liu, Wenling Han, Pingzhang Wang
(
BioLegend
,
302705
); anti-human CD49f-FITC (
BioLegend
,
313605
); and the fluorescent dye 7-AAD (
BioLegend
,
420404
). The cells were incubated with 1% Fc receptor blocker for 10 minutes and incubated with antibodies
(56)
Comprehensive Proteomics Analysis Identifies CD38-Mediated NAD+ Decline Orchestrating Renal Fibrosis in Pediatric Patients With Obstructive Nephropathy
Molecular & Cellular Proteomics
March 1, 2023
Yuandong Tao, Jifeng Wang, Xuexue Lyu, Na Li, Dong Lai, Yuanyuan Liu, Xingyue Zhang, Pin Li, Shouqing Cao, Xiaoguang Zhou,
et al.
(
103134
),
CD11b
FITC (
101206
),
Ly6G
APC/Cyanine7 (
127624
),
Ly6C
PE (
128008
),
F4/80
APC (
123116
), 7AAD (
420404
), and CD38 PE/Cyanine7 (
102717
).
Flow cytometry
was performed on a
FACSCanto
II (
BD Biosciences
), and
(57)
Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells
Cell reports
February 28, 2023
Wang C, Nistala R, Cao M, Pan Y, Behrens M, Doll D, Hammer RD, Nistala P, Chang HM, Yeh ETH,
et al
.
AB_2562339 Anti-hCD45-PE,
Biolegend
,Cat#
304039
; RRID: AB_2562057 Annexin V,
Biolegend
,Cat#
640941
; RRID: AB_2616657 7-AAD,
Biolegend
,Cat#
420404
PI,
Biolegend
,Cat#
421301
Bacterial and
virus
strains
,Bacterial and
virus
strains
,Bacterial and
virus
(58)
Intestinal dysbiosis exacerbates the pathogenesis of psoriasis-like phenotype through changes in fatty acid metabolism
Signal Transduction and Targeted Therapy
January 30, 2023
Qixiang Zhao, Jiadong Yu, Hong Zhou, Xiaoyan Wang, Chen Zhang, Jing Hu, Yawen Hu, Huaping Zheng, Fanlian Zeng, Chengcheng Yue,
et al.
for 30 min. Cellular viability was assessed by
staining
with 7-aminoactinomycin D (7-AAD) (
BioLegend
,
420404
) to exclude dead cells. For the analysis of cytokines production,
in vitro
re-stimulation and intracellular
(59)
Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile
iScience
January 20, 2023
Amanda N. Henning, Myagmarjav Budeebazar, Delgerbat Boldbaatar, Dahgwahdorj Yagaanbuyant, Davaadorj Duger, Khishigjargal Batsukh, Huizhi Zhou, Ryan Baumann, Robert D. Allison, Harvey J. Alter,
et al.
Tech,Cat#
17954
RNeasy Plus Micro kit,
Qiagen
,Cat#
74034
7-AAD Viability Stain Kit,
BioLegend
,Cat#
420403
TruSeq
RNA Library Prep
for Enrichment,Illumina,Cat# 20020189 TruSeq RNA Enrichment,Illumina,Cat#
(60)
Evolutionary history of transformation from chronic lymphocytic leukemia to Richter syndrome
Nature Medicine
January 9, 2023
Erin M. Parry, Ignaty Leshchiner, Romain Guièze, Connor Johnson, Eugen Tausch, Sameer A. Parikh, Camilla Lemvigh, Julien Broséus, Sébastien Hergalant, Conor Messer,
et al.
cat#
300330
using 2–4 uL of each antibody per 100 uL test) and a viability marker (
Biolegend
7-AAD cat#
420404
at
1:500
or Zombie
Violet
cat#
423114
at
1:1000
) before resuspension in PBS-0.04%
BSA
(Ultrapure
NEB
/Invitrogen).
Page
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Reviews
Write a Review
(1)
4/17/2023
Easy to Use Viability Staining Solution for Live and Dead Cell Detection
Soheil Tavakolpour
7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)
3/24/2023
Very Good 7AAD Viability Staining for Sorting
Im Hong Sun
Our lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ...
Read Review
(1)
7/7/2022
Easy to Use 7AAD Viability Stain
Matthew Ratsep
I use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ...
Read Review
(1)
3/13/2019
Staining Human T Cells with 7-AAD
Adam Guess
This reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ...
Read Review
(1)
8/3/2018
7AAD Works Well to Determine Viability by Flow Cytometry
Suzanne Tomchuck
In order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ...
Read Review
(1)
10/29/2015
Cell Viability Stain
Sachin Samuchiwal
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.
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7-AAD Viability Staining Solution from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
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Reviews
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